An Electrophoretic Study of LDHIsozyme Distribution in Developing Embryonic and Adult Tissues of the Toad Bufo arabicus

Lactate (LDH) dehydrogenases were studied by electrophoresis to investigate the possibility of isozymic differences in different stages and organs of B. arabicus embryos, tadpoles and adults. The expression pattern of lactate dehydrogenase (LDH) isozymes was analysed using nondenaturing discontinuous polyacrylamide gel electrophoresis (PAGE). Five LDH isozymes were observed in five different adult tissues. The most cathodal isozyme, LDH-5 (A4), showed the strongest activity. All embryonic stages tested displayed the anodic isozymes first. Four LDH isozymes were present in the stages 1-16 (fertilized egg-tail bud stage). The first appearance of the fifth isozyme was detected in stage 17 when the muscular response started. This isozyme showed gradual increase in intensity from stage 17 to stage 43.