Effect of Anti Bacterial Skin Secretion of IIRana ridibanda Frog on Methycillin Resistant Staph Aureus

The dorsal skin of the Iranian frog, Rana ridibanda, is associated with numerous prominent granular glands which extract their secretions in the response to stressor or invading pathogens .The secretions have broad spectrum antimicrobial effects. In this research the effect of antimicrobial skin secretions from Iranian frog (Rana ridibanda) has been examined against gram positive Methycillin Resistant Staph Aureus (MRSA), under sterile conditions. To show this effect, tern- of frog skin was cultured in complete tissue culture medium containing RPMI, FBS and FUNGISON for the period of 10 days. Immediately after, MRSA was exposed to frog skin secretion (medium culture) and Vancomycin. The results showed that the frog skin secretions has significant antimicrobial effect against MRSA. The range of inhibition zonefor MRSA was the sameas (20mm) Vancomycin in DISKmethod. In Minimum Inhibitory Concentration method, for MRSA, the tube 1/8 was positive.