Author/Authors :
Eleftheriadis, T. University of Thessaly - Medical School - Nephrology Department, Greece , Eleftheriadis, T. Theagenion Anticancer Hospital - Research Institute, Greece , Sparopoulou, T. Theagenion Anticancer Hospital - Research Institute, Greece , Antoniadi, G. University of Thessaly - Medical School - Nephrology Department, Greece , Liakopoulos, V. University of Thessaly - Medical School - Nephrology Department, Greece , Stefanidis, I. University of Thessaly - Medical School - Nephrology Department, Greece , Galaktidou, G. Theagenion Anticancer Hospital - Research Institute, Greece
Abstract :
Background and the purpose of the study: Indoleamine 2,3-dioxygenase (IDO) suppresses adaptive immune response. The purpose of this study was to determine the effect of the IDO inhibitor namely 1-methyl-DL-tryptophan (DL-1-MT) on antibody production after vaccination with hepatitis B surface (HBs) antigen.Methods: Four groups of BALB/c mice were immunized with a HBs antigen vaccine. In the first group the vaccine had no DL-1-MT, whereas in the other three groups the vaccine contained 1 mg , 10 mg and 20 mg DL-1-MT. Blood samples were collected 5 weeks post-vaccination and anti-HBs antibodies in the serum were measured by ELISA.Results: Compared to the three groups of mice that were immunized with the vaccines containing DL-1-MT, serum anti-HBs level was much higher in the mice that were immunized with the vaccine with out DL-1-MT.Conclusions: Inhibition of IDO at the time of vaccination decreased humoral immune response to HBs antigen vaccine. The idea that IDO activity is simply immunosuppressive may need to be re-evaluated
Keywords :
DL , 1 , MT% IDO% Serum anti , HBs%
