Author/Authors :
Habibi Roudkenar, M Iranian Blood Transfusion Organization - Research center, ايران , Mohammadi Roushandeh, A tabriz university of medical sciences - Faculty of Medicine - Department of Anatomical Sciences, تبريز, ايران , Mohammadi Roushandeh, A hamadan university of medical sciences - Faculty of Medicine - Department of Anatomical Sciences, همدان, ايران , Delazar, A. tabriz university of medical sciences - Faculty of Pharmacy - Department of Pharmacognosy, تبريز, ايران , Halabian, R. Iranian Blood Transfusion Organization - Research center, ايران , Soleimani Rad, J tabriz university of medical sciences - Faculty of Medicine - Department of Anatomical Sciences, تبريز, ايران , Mehdipour, A. tabriz university of medical sciences - Faculty of Medicine - Department of Anatomical Sciences, تبريز, ايران , Bagheri, M. Tohoku University - Graduate School of Medicine - Department of Pathology, Japan , Jahanian-Najafabadi, A. Pasteur Iinstitute of Iran - Department of Molecular Biology, تهران, ايران
Abstract :
Background and the purpose of the study: One of the most common malignancies in women is breast cancer. Although several treatments for breast cancer are available, application of herbal medicine as a supplementary treatment is a new option to help curing the disease. In this study anticancer effects of Polygonum avicular herbal extract was investigated.Methods: Polygonum avicular extract was obtained by methanol. MCF-7 cell line was treated with different concentrations of Polygonum avicular (50, 100, 150, 200, 250, 300,350 400 ng/μl) for different time lengths ( 6, 12, 24, and 48 hrs). MTT assay and Flow Cytometry were used to evaluate cell proliferation and apoptosis, respectively. RT-PCR was also carried out to evaluate the expression of apoptotic genes.Results and Discussion: Results showed that Polygonum avicular induced cytotoxicity in MCF-7 cell line at concentrations higher than 300 ng/μl and this was confirmed by the highest rate of cell death as measured by Trypan Blue and MTT assays. RT-PCR results showed up-regulation of P53 and down-regulation of Bcl-2 proteins which implied the ability of Polygonum avicular to induce apoptosis in MCF-7 cells and confirmed its anticancer property. Further studies are required to evaluate effects of the extract on other apoptotic genes
Keywords :
Herbal medicine , Breast cancer , knotweed , cell death
