Author/Authors :
Sollazzo, Vincenzo University of Ferrara - Orthopedic Clinic, Italy , Palmieri, Annalisa University of Bologna - Department of Histology, Embryology and Applied Biology, Italy , Scapoli, Luca University of Bologna - Department of Histology, Embryology and Applied Biology, Italy , Martinelli, Marcella University of Bologna - Department of Histology, Embryology and Applied Biology, Italy , Girardi, Ambra University of Bologna - Department of Histology, Embryology and Applied Biology, Italy , Pezzetti, Furio University of Bologna - Department of Histology, Embryology and Applied Biology, Italy , Morselli, Paolo University of Bologna - Plastic Surgery, Italy , Farinella, Francesca University of Ferrara - Department of Maxillofacial Surgery, Italy , Carinci, Francesco University of Ferrara - Department of Maxillofacial Surgery, Italy
Abstract :
Background: PerioGlas®(PG) is an alloplastic material used for grafting periodontal osseous defectssince 1995. In animal models, it has been proven that PG achieves histologically good repair of surgically created defects. In clinical trials, PG was effective as an adjunct to conventional surgery in the treatment of intrabony defects. Because the molecular events due to PG that are able to alter osteoblast activity to promote bone formation are poorly understood, we investigated the expression of osteoblastic related genes in mesenchymal stem cells exposed to PG. Methods: The expression levels of bone related genes like RUNX2, SP7, SPP1, COL1A1, COL3A1, BGLAP, ALPL, and FOSL1 and mesenchymal stem cells marker (CD105) were analyzed, using real time reverse transcription-polymerase chain reaction. Pearson s chi-square (χ 2) test was used to detect markers with significant differences in gene expression. Results: PG caused induction of osteoblast transcriptional factor (like RUNX2), bone related genes osteopontin (SPP1), osteocalcin (BGLAP) and alkaline phosphatase (ALPL). All had statistical significant P values ( 0.05). Conclusion: PG has a differentiation effect on mesenchymal stem cells derived from peripheral blood. The obtained results can be relevant to better understanding of the molecular mechanism of bone regeneration and as a model for comparing other materials with similar clinical effects.
Keywords :
Allograft , Alloplastic material , Bone , Stem cell.
