Inhibitory Effect of Antioxidants on Ferric-Nitrilotriacetic Acid Chelate Mediated Proxidation of Methyllinoleate

Antioxidants, namely mannitol, gallic acid, quercetin, α-tocopheryl acetate, and ascorbic acid, were evaluated for their inhibitory activities on the peroxidation of methyllinoleate (ML) induced by 1:2 ferric-nitrilotriacetic acid (Fe(III)(NTA)2) chelate. The thiobarbituric acid reactive substance (TBARS) assay was used to measure the inhibitory effect of the antioxidants (AOXs). The results of this study have revealed that Fe(III)(NTA)2-mediated peroxidation of ML was inhibited by each of the antioxidants used and the inhibition was concentration dependent. The decreasing order of inhibition of peroxidation of ML by the antioxidants at 1 mM concentration was found to be as follows: gallic acid quercetin mannitol tocopheryl acetate ascorbic acid. The probable causes of the observed decreasing order of inhibitory effect for the studied antioxidants have also been discussed.