Preparation and evaluation of molecularly imprinted polymer liquid chromatography column for the separation of Cathine enantiomers

In this study molecular imprinting technology was employed to prepare a specific affinity sorbent for the resolution of Cathine, a chiral drug product. The molecularly imprinted polymer (MIP) was prepared by non-covalent molecular imprinting with either (+) or ( #2;)-Cathine (threo-2-amino-1-hydroxy-1-phenyl propane; norpseudoephedrine) as the template. Methacrylic acid and ethylene glycol di-methacrylate were copolymerized in the presence of the template mol- ecule. The bulk polymerization was carried out in chloroform with 2,20-azobisisobutyronitrile as the initiator, at 5 #2;C and under UV radiation. The resulting MIP was ground into powders, which were slurry packed into analytical columns. After removal of template molecules, the MIP-packed columns were found to be effective for the resolution of (±)-Cathine racemates. The separation fac- tor for the enantiomers ranged between 1.5 and 2.4 when the column was packed with MIP pre- pared with (+)-Cathine as the template. A separation factor ranging from 1.6 to 2.9 could be achieved from the column packed with MIP, prepared with (-)-Cathine as the template. Although the separation factor was higher with that previously obtained from reversed-phase column chro- matography following derivatization with a chiral agent, elution peaks were broader due to the het- erogeneity of binding sites on MIP particles and the possible non-specific interaction.