BIOSYNTHESIS OF ANTIBIOTIC THROUGH METABOLISM OF ACTINOMYCETES STRAIN MH-9 THROUGH SHAKE FLASK FERMENTATION

A strain of MH-9 (Actinomycetes) was isolated from desert soil and manipulated to detect its ability to produce antibiotics. Optimization of different parameters reflected that the antibiotic production was maximum at pH 7 and 37°C. The optimum antibiotic production was obtained after 120- hours of incubation at 150 rpm. Antibiotic activity was recorded as maximum i.e. 38mm against Bacillus subtilis, while increase of E.coli, Pseudomonas and Staphylococcus aureus zone of 30,30 and 31mm were shaped respectively. Extraction of sample with different solvents was carried out and antibiotic which was synthesized as a result of metabolic activities of MH-9 was found to be water soluble. The effect of proteins on antibiotic was observed by column chromatography and noted the activity in different fractions. Concentration for streptomycin (4473μg/ml) and (228μg/ml) were determined in the fractions and further analysis was confirmed by IR spectrophotometery and TLC method. Moreover the activity of crude antibiotic mixture with the standard streptomycin and kanamycin was also compared. Antibiotic Activity was also checked and was found best against Gram-positive and found good against Gram-negative bacteria.