Author/Authors :
ABDULLAH, JAAFAR Universiti Kebangsaan Malaysia - Fakulti Sains dan Teknologi - Pusat Pengajian Sains Kimia dan Teknologi Makanan, Malaysia , AHMAD, MUSA Universiti Kebangsaan Malaysia - Fakulti Sains dan Teknologi - Pusat Pengajian Sains Kimia dan Teknologi Makanan, Malaysia , HENO, LEE YOOK Universiti Kebangsaan Malaysia - Fakulti Sains dan Teknologi - Pusat Pengajian Sains Kimia dan Teknologi Makanan, Malaysia , KARUPPIAH, NADARAJAH SIRIM Berhad - Pusat Alam Sekitar dan Teknologi Bioproses, Malaysia , SIDEK, HAMIDAH SIRIM Berhad - Pusat Alam Sekitar dan Teknologi Bioproses, Malaysia
Abstract :
An optical biosensor based on immobilized tyrosinase in sol-gelfilmfor phenol detection is described in this paper. The film oftyrosinaselsol-gel was prepared by smearing technique on a plastic slide support. The o-quinone resulted from the enzymatic oxidation of phenol was reacted with 3-methyl-2-benzothiazolinone hydrazone (MBTH) to produce a maroon colour adduct that can be observed at 490 nm. The linearity of the biosensor towards phenol was found to be in concentration range of 0.031 mM to 0.25 mM with detection limit of 0.003 mM. The RSD of the biosensor fabrication studies was 8.8 %.
Keywords :
Phenol , tyrosinase enzyme , sol , gel film , optic
