Author/Authors :
Zainal Ariffin, Shahrul Hisham Universiti Kebangsaan Malaysia - Fakulti Sains dan Teknologi - Pusat Pengajian Biosains dan Bioteknologi, Malaysia , Nordin, Noreena Universiti Kebangsaan Malaysia - Fakulti Sains dan Teknologi - Pusat Pengajian Biosains dan Bioteknologi, Malaysia , Foo, Siah Li Universiti Kebangsaan Malaysia - Fakulti Sains dan Teknologi - Pusat Pengajian Biosains dan Bioteknologi, Malaysia , Juliana Ariffin, Eni Universiti Kebangsaan Malaysia - Fakulti Sains dan Teknologi - Pusat Pengajian Biosains dan Bioteknologi, Malaysia , Senafi, Sahidan Universiti Kebangsaan Malaysia - Fakulti Sains dan Teknologi - Pusat Pengajian Biosains dan Bioteknologi, Malaysia , Abdul Wahab, Rohaya Megat Universiti Kebangsaan Malaysia - Fakulti Pergigian - Jabatan Ortodontik, Malaysia
Abstract :
Type I collagen a-2 gene is a member of collagen group that can be found in pulp tissues. Type I collagen constitutes about 56% of large intercellular spaces in the pulp. The objective of this study is to isolate the Col1A2 gene, directly from RNA isolation from pulp tissue Orycytolagus cuniculus. A housekeeping gene, i.e., GAPDH is used as a positive control gene. Specific primer sets for Col12 and GAPDH genes have been designed using Primer Premier Version 5 based on both gene sequences obtained from NCBI (National Center for Biotechnology Information) [www.ncbi.nlm.nih.gov]. RNA had been isolated directly from pulp tissues of Oryctolagus cuniculus. The PCR products for Col1A2 and GAPDH were successfully amplified with the size of 893 bp (Col1A2) and 229 bp (GAPDH). Sequencing results had shown that Col1A2 and GAPDH clone attained almost 99% similarity and 100% identical compared to the sequence in the NCBI database respectively. This study shows that Col1A2 gene has been successfully detected from pulp tissue of O. cuniculus.
Keywords :
Odontoblast , dentine , pulp Tissues , Oryctolagus cuniculus , RT , PCR
