Title of article :
A Simple HPLC Method for Quantitation of Diacerein in Tablet Dosage Form
Author/Authors :
Lalitha, Keddal G. Ultra College of Pharmacy - Department of Pharmaceutical Chemistry, India , Venkatachalam, Thangavel Annai JKK Sampoorani Ammal College of Pharmacy - Department of Pharmaceutical Chemistry, India , Srinivasan, Ramaraj Annai JKK Sampoorani Ammal College of Pharmacy - Department of Pharmaceutical Chemistry, India , Kalaiselvi, Ponnusamy Annai JKK Sampoorani Ammal College of Pharmacy - Department of Pharmaceutical Chemistry, India , Kannappan, Nagappan Annamalai University - Faculty of Engineering Tecnology - Department of Pharmacy, India
Abstract :
A simple, economic, accurate reverse phase isocratic RP-HPLC method has been developed for the quatitation of diacerein in tablet dosage form. The quantitation was carried out using Zorbax CN column. The mobile phase was ammonium acetate buffer (pH adjusted to 3.5) : Acetonitrile [53:47]. The LOD and LOQ are found to be 3.952 μg mL^-1 and 11.97 μg mL^-1 respectively. The flow rate was 1mL/min with UV detection at 254 nm.The method has been validated and proved to be accurate, precise, linear, rugged, robust, simple and rapid. The calibration curve was linear in the concentration range 25-150 μg mL^-1 with coefficient of correlation 0.99942. The percentage recovery of diacerein was found to be 101.60%.The method is useful in the quality for the estimation of diacerein in tablet dosage form.
Keywords :
Diacerein , RP , HPLC , Tablets , Validation
Journal title :
eurasian journal of analytical chemistry
Journal title :
eurasian journal of analytical chemistry
