Author/Authors :
bankey, s.k. school of pharmaceutical sciences,rajiv gandhi technological university,gandhi nagar,bhopal, India , jain, s.k. sagar institute of research and technology,bhopal,462 036, India , basniwal, p.k. lbs college of pharmacy,jaipur,rajasthan, India , jain, d. school of pharmaceutical sciences,rajiv gandhi technological university,gandhi nagar,bhopal, India
Abstract :
A simple,accurate and precise HPLC method was developed and validated for determination of rimonabant in human plasma. Following liquid-liquid extraction,chromatographic separation was accomplished using C18 column with mobile phase consisting of acetonitrile: water (90:10,v/v),drug was detected at 260 nm. The LOD and LOQ were 3.3 and 9.9 ng mL-1,respectively. The method is linear in range 50-1000 ng mL-1. The average extraction recovery of drug from plasma was found 78.45%. The precision of method was found less than 2.8%,and accuracy was found between 97.12-101.71%. The assay may be applied to a pharmacokinetic and bioequivalence study of rimonabant. © 2012 Moment Publication.
Keywords :
Human plasma , Pharmacokinetic study , Rimonabant , RP , HPLC
