Author/Authors :
Zain, S.M. Universiti Kebangsaan Malaysia - Faculty of Engineering Built Environment - Department of Civil Structural Engineering, Malaysia , Hashim, R. Universiti Kebangsaan Malaysia - Faculty of Engineering Built Environment - Department of Civil Structural Engineering, Malaysia , Roslani, N.S. Universiti Kebangsaan Malaysia - Faculty of Engineering Built Environment - Department of Civil Structural Engineering, Malaysia , ANUAR, N. Universiti Kebangsaan Malaysia - Faculty of Engineering Built Environment - Department of Chemical Process Engineering, Malaysia , SUJA, F. Universiti Kebangsaan Malaysia - Faculty of Engineering Built Environment - Department of Civil Structural Engineering, Malaysia , Daud, W.R.W. Universiti Kebangsaan Malaysia - Faculty of Engineering Built Environment - Department of Chemical Process Engineering, Malaysia , Basri, N.E.A. Universiti Kebangsaan Malaysia - Faculty of Engineering Built Environment - ment of Civil Structural Engineering, Malaysia
Abstract :
This study aimed to determine the types of bacteria exist in wastewater that contibute to generate electricity and simultaneously remove carbon and nitrogen. The method used was Fluorescence In Situ Hibridization (FISH) to detect the bacteria group while Polymerase Chain Reaction (PCR) was used to confirm the observation made using FISH. A biochemical identification using BIOLOG GEN III MICROPLATE™ also was used . The samples were cultured on nutrient agar plate to identify the morphology of the bacteria. The result showed that 21 isolates from three different locations at the activated sludge treatment plant with six, eleven and four strains for raw sewage, aeration tank and returned activated sludge samples, respectively. Preliminary identification does not give a good match but only showed the existence of ammonia-oxidizing bacteria (FISH) and Kurtia Gibsoni (BIOLOG) from aeration tank : Bacillus sp (PCR) and Bacillus Pseudomycoides (BIOLOG) from returned activated sludge. The maximum power density generated using returned activated sludge was 9.053 mW/cm2, with 26.8% COD removal and 40% TKN removal
Keywords :
Bacteria , Fluorescence In Situ Hibridization(FISH) , microbial fuel cell , polymerasa chain reaction(PCR) , sewage
