Kesan ß-gliserofosfat Terhadap Ampaian Sel Mononukleus daripada Darah Periferi Manusia

Osteoblast cell is a mononucleus cell that is responsible for bone formation. Mononucleus cell have been proven to differentiate into osteoblast cell after being induced with combinations of ascorbic acid and ß-glycerophosphate as differentiation factors. This study aimed to observe the in vitro effect of a single ß-glycerophosphate induction towards primary mononucleated cells suspension that have been isolated from human peripheral blood. Mononucleus cells were isolated from human peripheral blood using density gradient centrifugation method in the present of Ficoll-Paque™ Plus. Mononucleus cells were cultured for 7 days in proliferation medium before being induced by 1 mM, 5 mM, 10 mM, 20 mM, and 100 mM of ß-glycerophosphate. Alkaline phosphatase (ALP) activity profile and morphological analysis were done at day 0, 1, 3, 7 and 14 in their respective medium. The activity of ALP enzyme and morphology analysis showed a significant increase (p 0.05) between cells that have been induced with the negative control cells by using paired t-test statistical analysis. In conclusion, the presence of only ß-glycerophosphate is able to induce mononucleated cell differentiation into osteoblast cells. The effect of ß-glycerophosphate towards mononucleated cells differentiation showed similar enzymological (ALP activities) and morphological profile as positif control (ascorbic acid and ß-glycerophosphate). Based on enzymological and morphological analyses, 1 mM of ß-glycerophosphate is the best concentration for in vitro osteoblast differentiation