Different frequency of epidermal growth factor rs76189946 polymorphism genotype in Iranian colorectal cancer patients

Aim: This study aimed to determine the association between rs76189946 polymorphism of EGF gene and the risk of colorectal cancer in an Iranian population. Background: Colorectal cancer (CRC) is the third most prevalent cancer in both genders worldwide. The determination of genetic variation becomes a new way to the etiology of colorectal cancer. Epidermal growth factor (EGF) is a mitogen that plays an important role in cell growth and tumourigenesis. This protein acts by binding its receptor, EGFR. Patients and methods: DNA samples taken from totally 125 CRC patients and healthy controls were amplified by polymerase chain reaction (PCR) for the rs76189946 polymorphism. Genotypes were analyzed using restriction fragment length polymorphism (RFLP). Finally to confirm the RFLP procedure, 20 of the PCR products were sequenced using the ABI PRISM 3130xl genetic analyzer and chain termination method (Applied Biosystems, Carlsbad, CA). Results: Genotype distribution and allele frequency was similar in CRC patients and controls individuals. We expect to observe C and G allele in both groups, but only found C allele. Conclusion: In this study for the first time we identified genetic distribution of exonic rs76189946 polymorphism in EGF gene both CRC patients and healthy controls. These results suggest there was no association between EGF polymorphism rs76189946 and risk of colorectal cancer in an Iranian population. © 2013 RIGLD.