Effect of Apigenin, Berberine and Rutin on Cholesterol Metabolism in Hep G2 Cancer Cell

In this study, the ability of Apigenin, Berberine and Rutin to reduce cholesterol metabolism in human hepatoma cancer cell line (Hep G2) was determined. Cytotoxic assessment of Apigenin, Berberine and Rutin were performed by exposing the Hep G2 to Apigenin, Berberine and Rutin at concentrations ranging from 7.8 to 1000 μg/mL for 24 h at 37oC and with 5% CO2 atmosphere. The inhibition concentration 20 (IC20) of Apigenin, Berberine and Rutin were 7.8, 125 and 1000 μg/mL, respectively. The ability of reducing cholesterol metabolism effects of Apigenin, Berberine and Rutin on Hep G2 was carried out by seeding the cell in 6-well plates. In the treated groups, Hep G2 was treated with Apigenin, Berberine and Rutin (7.8, 31.25 and 62.5 μg/mL, respectively) and exposed to 10 μL low density lipoprotein (LDL). In the normal control (NC) groups, Hep G2 was incubated with culture medium only. The cell was incubated and media was taken for analysis of Apo A1, LCAT, LDLR and FDFT1 by using kit. Rutin was able to lower down HMGR activity significantly (p 0.05) compared with normal control. Apigenin and Berberin were able to increase the concentration of APO A1. All the three samples can increase the concentration of LCAT in treated cells. In addition, Apigenin can increase the concentration of LDLR. The test results for FDFT1 showed that Berberin and Rutin can lower down FDFT1 concentrations and significantly different (p 0.05) compared with normal controls. These findings suggested that Apigenin, Berberine and Rutin have the potential in reducing cholesterol metabolism in Hep G2 cancer cell lines.