Author/Authors :
YIP, WU HON Universiti Kebangsaan Malaysia - Faculty of Science and Technology, School of Chemical Sciences and Food Technology, Malaysia , JOE, LIM SENG Universiti Kebangsaan Malaysia - Faculty of Science and Technology, School of Chemical Sciences and Food Technology, Malaysia , WAN MUSTAPHA, WAN AIDA Universiti Kebangsaan Malaysia - Faculty of Science and Technology, School of Chemical Sciences and Food Technology, Malaysia , MASKAT, MOHAMAD YUSOF Universiti Kebangsaan Malaysia - Faculty of Science and Technology, School of Chemical Sciences and Food Technology, Malaysia , SAID, MAMOT Universiti Kebangsaan Malaysia - Faculty of Science and Technology, School of Chemical Sciences and Food Technology, Malaysia
Abstract :
This study was carried out to identify the pigment extracted from Malaysian brown seaweed, Sargassum binderi and its stability in various conditions. Pigments were extracted using methanol:chloroform:water (4:2:1, v/v/v), which is part of fucoidan extraction process, where the pigments were waste. Carotenoid and chlorophyll were found in the extract using UV-vis spectrophotometer (420 and 672 nm, respectively). Fucoxanthin was identified as the carotenoid present using HPLC, while its functional groups and structure were determined using FTIR and 1H NMR, respectively. The fucoxanthin-rich extract stability was tested on different pH (pH1-13), light exposure (dark and light) and storage temperature (4°C, 25°C and 50°C). The stability tests showed that it was most stable at pH5-7, stored in dark condition and at the storage temperature of 4°C and 25°C. The fucoxanthin-rich extract from Sargassum binderi has potential to be applied as bioingredient and functional food as it is stable in normal storage conditions.
Keywords :
Carotenoid , chlorophyll , fucoxanthin , pigment , Sargassum binderi
