Title of article :
Rhizopus Species from Fresh Tempeh Collected from Several Regions in Indonesia
Author/Authors :
Hartanti, Anastasia Tatik Bogor Agricultural University, Darmaga Campus - Faculty of Mathematics and Natural Sciences - Department of Biology, Indonesia , Hartanti, Anastasia Tatik university of indonesia - Faculty of Biotechnology - Department of Biology, Indonesia , Rahayu, Gayuh Bogor Agricultural University, Darmaga Campus - Faculty of Mathematics and Natural Sciences - Department of Biology, Indonesia , Hidayat, Iman Indonesian Institute of Sciences (LIPI) - Research Center for Biology, Indonesia
Abstract :
Recent changes in taxonomy of Rhizopus, which are now heavily relying on molecular approach, create significant problem in assigning species name to particular Rhizopus strains isolated from various sources, including tempeh. The present study aims to determine 36 strains of Rhizopus from tempeh originated from 26 locations in Indonesia, using combination of molecular phylogenetic analysis based on internal transcribed spacer ribosomal DNA sequence, physiology, and morphology to species level. The results showed that most of the strains belong to R. microsporusecomplex, and only one strain belongs to R. delemar. Morphological variations within R. microsporus were observed, but under current approach they were insufficient for infraspecific delimitation. The current report is an important contribution in validating the identity of Rhizopus from fresh tempeh in Indonesia.
Keywords :
ITS rDNA , Rhizopus , taxonomy , tempeh
Journal title :
HAYATI Journal of Biosciences
Journal title :
HAYATI Journal of Biosciences
