Author/Authors :
Sharafi, Heidar Armin Pathobiology Laboratory, ايران , Sharafi, Heidar Tehran Hepatitis Cohort (THC) Study Center, ايران , Pouryasin, Ali Armin Pathobiology Laboratory, ايران , Pouryasin, Ali Tehran Hepatitis Cohort (THC) Study Center, ايران , Pouryasin, Ali islamic azad university - Department of Genetics, ايران , Alavian, Moayed Tehran Hepatitis Cohort (THC) Study Center, ايران , Alavian, Moayed baqiyatallah university of medical sciences - Baqiyatallah Research Center for Gastroenterology and Liver Diseases, ايران , Behnava, Bita baqiyatallah university of medical sciences - Baqiyatallah Research Center for Gastroenterology and Liver Diseases, ايران , Behnava, Bita Tehran Hepatitis Cohort (THC) Study Center, ايران , Keshvari, Maryam Iranian Blood Transfusion Organization, ايران , Keshvari, Maryam Tehran Hepatitis Cohort (THC) Study Center, ايران , Salimi, Shima Tehran Hepatitis Cohort (THC) Study Center, ايران , Salimi, Shima baqiyatallah university of medical sciences - Baqiyatallah Research Center for Gastroenterology and Liver Diseases, ايران , Mehrnoush, Leila Tehran Hepatitis Cohort (THC) Study Center, ايران , Mehrnoush, Leila baqiyatallah university of medical sciences - Baqiyatallah Research Center for Gastroenterology and Liver Diseases, ايران , Fatemi, Ahmad tehran university of medical sciences tums - Department of Hematology, تهران, ايران
Abstract :
populations, respectively. A few studies reported that there is a mysterious association between the IL28B polymorphism and the hepatitis C virus (HCV) genotype in patients with chronic hepatitis C but they did not give any reason for this phenomenon. Objectives: The foremost purpose of this study was to compare the distribution of IL28B genotypes between Iranian healthy individuals and patients with chronic hepatitis C. Patients and Methods: In this study, 921 patients with chronic hepatitis C and 142 healthy individuals were included. The IL28B rs12979860 and rs8099917 polymorphisms were genotyped using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Results: The frequency of IL28B rs12979860 CC, CT, and TT genotypes in chronic hepatitis C patients was 38%, 48.8%, and 13.2% and in healthy individuals was 43.7%, 48.6%, and 7.7%. Also, the frequency of IL28B rs8099917 TT, GT, and GG genotypes in chronic hepatitis C patients was 58.3%, 37.1%, and 4.6% and in healthy individuals was 64.1%, 32.4% and 3.5%. The differences in the distribution of IL28B rs12979860 and rs8099917 genotypes between patients with chronic hepatitis C and healthy individuals were not statistically significant. When we compared the distribution of IL28B genotypes between the healthy group and the HCV infected patients by HCV genotype, we found 9.8% higher frequency of rs12979860 CC genotype in the healthy individuals than HCV genotype 1 infected patients (P = 0.03) however there was no significant difference in the distribution of rs12979860 genotypes between the healthy and HCV genotype 3 infected groups (P = 0.46). Conclusions: It seems that the impact of IL28B polymorphism on the spontaneous clearance of HCV genotype 1 is more prominent than HCV genotype 3 which results in the observation of higher rs12979860 C allele frequency in chronic hepatitis C patients with HCV genotype 3 than HCV genotype 1.
Keywords :
Polymorphism, Genetic , Hepatitis C , IL28B Genotype, Human , Persian Gulf , Iran
