Author/Authors :
Chen, Qin-Yan Guangxi Zhuang Autonomous Region Center for Disease Prevention and Control, China , Harrison, Tim J University College London - Faculty of Medical Sciences - Division of Medicine, UK , Sabin, Caroline A UCL Medical School - Research Department of Infection and Population Health, UK , Li, Guo-Jian Department of Public Health of Guangxi Zhuang Autonomous Region, China , Huang, Gao-Ming Guangxi Medical University - school of Public Health, China , Yang, Jin-Ye Guangxi Zhuang Autonomous Region Center for Disease Prevention and Control, China , Wang, Xue-Yan Guangxi Zhuang Autonomous Region Center for Disease Prevention and Control, China , Li, Hai Guangxi Zhuang Autonomous Region Center for Disease Prevention and Control, China , Liu, Mo-Han Guangxi Zhuang Autonomous Region Center for Disease Prevention and Control, China , Liu, Mo-Han Guangxi Medical University - School of Preclinical Medicine, China , Fang, Zhong-Liao Guangxi Zhuang Autonomous Region Center for Disease Prevention and Control, China , Fang, Zhong-Liao Guangxi Medical University - School of Preclinical Medicine, China
Abstract :
Background: Association of hepatitis B virus (HBV) genotype C with hepatocellular carcinoma (HCC) development remains controversial. HBV basal core promoter (BCP) double mutations (T1762A1764) are very strong confounding factors of genotypes B and C in HCC development.Objectives: To investigate the association of HBV genotype C with HCC development after controlling for BCP double mutations. Materials and methods: Four hundred and two serum samples from patients with HCC, liver cirrhosis (LC) and chronic hepatitis (CH) and also from asymptomatic HBsAg carriers were analyzed. Results: Genotypes B (31.1%), C (62.8%), and I (6.1%) were detected. With the severity of liver disease the prevalence of genotype B decreased, but genotype C increased. No trend was found for genotype I. The prevalence of BCP double mutations in genotypes C and I viruses was significantly higher than genotype B. BCP double mutations are risk factors for CH, LC and HCC. Genotype C was not identified as a particular risk factor for HCC prior to the stratification analysis but after that genotype C viruses with BCP double mutations were found to be a particular risk factor for HCC (P = 0.008, OR = 17.19 [95% CI: 2.10 - 140.41]), but those with the wild-type BCP were not. In the interaction analysis, genotype C and BCP double mutations were found to have a synergistic effect on HCC development (P 0.0001, OR = 52.56 [95% CI: 11.49-240.52]). Conclusions: The effect of HBV genotype C on the development of HCC differs between wild-type viruses and those with BCP double mutations, suggesting that not all individuals infected with genotype C HBV are at increased risk of HCC.
Keywords :
Hepatitis B Virus , Genotypes , Basal Core Promoter Double Mutations , Hepatocellular Carcinoma
