Author/Authors :
Grauda, Dace University of Latvia - Institute of Biology, LATVIA , Mikelsone, Andra University of Latvia - Institute of Biology, LATVIA , Lisina, Nadezda University of Latvia - Institute of Biology, LATVIA , Zagata, Karlis University of Latvia - Institute of Biology, LATVIA , Ornicans, Reinis University of Latvia - Institute of Biology, LATVIA , Fokina, Oksana University of Latvia - Institute of Biology, LATVIA , Lapina, Lita University of Latvia - Institute of Biology, LATVIA , Rashal, Isaak University of Latvia - Institute of Biology, LATVIA
Abstract :
Our goal was to improve the method of obtaining double haploids (DH) by anther culture from Latvian breeding material for several agriculturally important cereals in Latvia: barley, spring and winter wheat. Hybrids from Latvian breeders were initial material. It was found that copper (2.5 mg/l) added to barley anther pre-treatment media and to both barley (C3) and wheat (liquid AMC) induction media, resulted in a higher percentage of green plants-regenerants. In general, the winter wheat hybrids exhibited lower embryogenesis than spring wheat hybrids. Obtaining a large number of DH lines from barley and wheat hybrids with unknown androgenesis response can be organized in two stages: first — selection from breeding initial material hybrids responsive in anther culture, which enables production of green plants-regenerants, and second — production of DH lines in a large scale from selected hybrids.
Keywords :
DH lines , breeding , barley , wheat , copper