Title of article
Study on the Interaction of 4'-Hydroxychalcones and their Mannich Derivatives with Calf Thymus DNA by TLC and Spectroscopic Methods, a DNA Cleavage Study
Author/Authors
Rozmer, Zsuzsanna Institute of Pharmaceutical Chemistry - University of Pécs, Rókus str. 2., Hungary , Bernardes, Aline Institute of Chemistry - Federal University of Goiás, 74690-900 Goiánia-GO, Brazil , Pérez, Caridad N. Institute of Chemistry - Federal University of Goiás, 74690-900 Goiánia-GO, Brazil , Perjési, Pál Institute of Pharmaceutical Chemistry - University of Pécs, Rókus str. 2., Hungary
Pages
10
From page
122
To page
131
Abstract
Background:
Phenolic Mannich bases derived from hydroxychalcones show remarkable cytotoxic potencies towards cancer cell lines. However, the exact mechanism of action is still partially uncleared.
Objective:
Interaction of two hydroxychalcones and their Mannich derivatives with calf thymus DNA (ctDNA) has been investigated.
Methods:
Thin-layer chromatography and UV-Vis spectroscopic method were used for studying the interaction. The binding constant has been determined by UV-Vis spectrophotometric titration. The DNA cleavage activity of the compounds was studied by agarose gel electrophoresis.
Results:
Interaction of the compounds with ctDNA exhibited relatively high intrinsic binding constant (4-5x104 M-1). The results indicate existence of weak, non-covalent interactions between the investigated derivatives with ctDNA. Some compounds showed a slight DNA cleavage activity with pBR322.
Conclusion:
The obtained results provide additional knowledge on the previously documented cytotoxicity against tumor cell lines of the hydroxychalcones and their Mannich-derivatives.
Farsi abstract
فاقد چكيده فارسي
Keywords
Chalcones , Hydroxychalcones , Mannich bases , DNA binding , UV-Vis spectroscopy , DNA cleavage
Journal title
Open Medicinal Chemistry Journal
Serial Year
2020
Full Text URL
Record number
2559606
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