Author/Authors :
Zulkifli, Y. Universiti Putra Malaysia - Faculty of Biotechnology and Biomolecular Sciences - Department of Cell and Molecular Biology, Malaysia , Alitheen, N. B. Universiti Putra Malaysia - Faculty of Biotechnology and Biomolecular Sciences - Department of Cell and Molecular Biology, Malaysia , Son, R. Universiti Putra Malaysia - Faculty of Food Science and Technology, Centre of Excellence for Food Safety Research, Malaysia , Yeap, S. K. Universiti Putra Malaysia - Faculty of Biotechnology and Biomolecular Sciences - Department of Cell and Molecular Biology, Malaysia , Lesley, M. B. Universiti Malaysia Sarawak - Faculty of Resource and Science Technology - Department of Molecular Biology, Malaysia , Raha, A. R. Universiti Putra Malaysia - Faculty of Biotechnology and Biomolecular Sciences - Department of Cell and Molecular Biology, Malaysia
Abstract :
Vibrio parahaemolyticus is a gram negative bacterium and causes gastrointestinal illness in humans. In this study, twenty five out of fifty cockle samples from Padang, Indonesia produced purple colonies when they were grown on selective medium, CHRO Magar^TM Vibrio. Specific–PCR for toxR gene detection gave positive results in which a band with 368 base pairs size appeared on the gel for all the isolates that confirmed the presence of V. parahaemolyticus. In the virulence properties test, all the isolates showed negative results for tdh and trh genes detection. The results indicate that the isolates under this study do not contain virulence properties that correlate to the ability of infection and diseases, which means that they are nonpathogenic.
Keywords :
Vibrio parahaemolyticus , toxR gene , PCR , trh gene , tdh gene
