Author/Authors :
Sahilah, A.M. Universiti Kebangsaan Malaysia - Faculty of Science and Technology, School of Chemical Sciences and Food Technology, Institute of West Asian Studies (IKRAB), Malaysia , Norhayati, Y. Universiti Kebangsaan Malaysia - Faculty of Science and Technology, School of Chemical Sciences and Food Technology, Malaysia , Norrakiah, A. S. Universiti Kebangsaan Malaysia - Faculty of Science and Technology, School of Chemical Sciences and Food Technology, Malaysia , Aminah, A. Universiti Kebangsaan Malaysia - Faculty of Science and Technology, School of Chemical Sciences and Food Technology, Malaysia , Wan Aida, W. M. Universiti Kebangsaan Malaysia - Faculty of Science and Technology, School of Chemical Sciences and Food Technology, Malaysia
Abstract :
Chicken (Gallus gallus), cattle (Bos taurus), goat (Capra hircus), pig (Sus scrofa domestica) and wild boar (Sus scrofa linneus) raw meats were examined using PCR amplification of mitchondrial DNA. Three oligoprimers were used to amplified mitochondria DNA (mtDNA) of cytochrome b (two types of primers) and mitochondrial 12s rRNA (mt-12s rDNA) (one type of primer) gene for vertebrate-specific. The amplification PCR products of 359 bp and 531 bp were successfully amplified from the cyt b gene of pig and wild boar meats using two type of mtDNA. None of the band was observed for chicken, cattle and goat. While, the amplification product of all meats using mt-12S rDNA gene were successfully produced a single band with molecular size of 456 bp, which were as expected due to all animals were veterbrate-specific. Thus, this primer could not be used to detect the pig DNA in raw meat samples. In the present work, the PCR amplification of mtDNA of cytochrome b has been shown as a suitable tool for rapid detection of pig DNA in foods.
