Title of article :
Differential scanning calorimetry as tool in observing thermal and storage stability of recombinant bromelain
Author/Authors :
Nurul, A. I. International Islamic University Malaysia - Kuliyyah of Engineering - Department of Biotechnology Engineering, Biomolecular Engineering Research Group, Malaysia , Azura, A. International Islamic University Malaysia - Kuliyyah of Engineering - Department of Biotechnology Engineering, Biomolecular Engineering Research Group, Malaysia
Abstract :
Knowledge about the thermal and storage behavior of produced protein is important for the purpose of storage, transport and shelve life during industrial application. Recombinant bromelain thermal and storage stability were measured and compared to the commercial bromelain using Differential Scanning Calorimetry (DSC). Recombinant bromelain is more stable than commercial bromelain at higher temperature but the stability was reduced after 7 days of storage at 4°C. Higher energy is needed to break the bond between amino acid chains in recombinant bromelain as shown by the enthalpy obtained, suggesting that recombinant bromelain has good protein structure and conformation compared to commercial.
Keywords :
DSC , thermal stability , storage stability , recombinant bromelain
Journal title :
International Food Research Journal
Journal title :
International Food Research Journal
