Rapid and sensitive HPLC-PDA method for simultaneous identification and quantification of dietary weight reducing compound hydroxy citric acid lactone and chemo preventive compounds isoxanthochymol and xanthochymol in Garcinia indica

Following optimization of extraction, separation and analytical conditions, a rapid, sensitive and simple reverse-phase HPLC-PDA method has been developed for the simultaneous identification and quantification of hydroxy citric acid lactone (HCAL), isoxanthochymol (Isoxan) and xanthochymol (Xan) in different extracts of Garcinia indica (Guttiferae). The separation of HCAL, Isoxan and Xan was achieved on a Waters Sunfire C-18 column using the solvent system consisting of a mixture of acetonitrile - water (90:10, v/v) and methanol - acetic acid (99.5:0.5, v/v) as a mobile phase followed by photo diode array (PDA) detection of peaks at 220 and 276 nm. Calibration curves presented good linear regression (r² 0.986) within the test ranges.