Title of article :
The phenolic contents and antiradical activity of Indonesian Phyllantus urinaria L.
Author/Authors :
Arina, N. B. Gadjah Mada University - Faculty of Pharmacy - Research Center of Halal Products, Indonesia , Abdul Rohman Gadjah Mada University - Faculty of Pharmacy - Research Center of Halal Products, Indonesia , Abdul Rohman Universiti Teknologi Malaysia (UTM) - Centre of Research For Fiqh Science Technology (CFiRST), Malaysia
Abstract :
In this study, the 2,2-diphenyl-1-picrilhydrazil (DPPH) antiradical activity as well as the phenolics and flavonoid contents of ethanolic extract and its fraction of Phyllantus urinaria L. was evaluated. The ethanolic extract of P. urinaria L. was fractionated using vaccum column chromatography and gradient elution. Based on TLC profile, the fractination process yielded 6 fractions. The ethanolic extract of P. urinaria L. showed the high DPPH free radical scavenging with IC50 of 5.74 μg/mL. Among 6 fractions, fraction 6 had the highest DPPH free radical scavenging activity with IC50 of 4.22 μg/mL. Among ethanol extract and its fractions evaluated, fraction 5 had the highest phenolics and flavonoid contents. The correlation between DPPH free radical scavenging activities and phenolics contents existed with the equation of y = -43.454x + 827.67 with coefficient of determination (R²) of 0.3475, while, the R² for the relationship between IC50 values and flavonoid contents of ethanolic extract and its fraction is 0.2325.
Keywords :
DPPH free radical , scavenging activity , Phyllantus urinaria L. , phenolic contents , flavonoid contents
Journal title :
International Food Research Journal
Journal title :
International Food Research Journal
