Performance of starch hydrolysis and production of corn syrup using some commercial enzymes

Five commercial amylolytic enzyme preparations, two liquefying (Termamyl Supra and Clarase L40, 000) and three saccharifying (AMG E, Dextrozyme DX and Optimax 4060 VHP) were used for the performance of starch hydrolysis to produce corn syrup. The operating conditions of these preparations showed that AMG E was the least effective enzyme within the saccharifying enzymes tested. Four enzyme combinations from the other four liquefying and saccharifying enzymes were tested for the starch hydrolysis. The results indicate that the liquefaction period by Termamyl S and Clarase must not exceed than 90 min whereas, the best starch concentrations were 30 gdl-1 for Termamyl S and 40 gdl-1 for Clarase. It was found that the combinations of Clarase followed by Optimax or Dextrozyme were more effective than those of Termamyl S, followed by the same two enzymes. The carbohydrate profile of the produced corn syrup showed that glucose is the main component (86.92%).The values of dextrose equivalent (DE) and the true dextrose equivalent (DX) of corn syrup were 79.587 and 85.334, respectively.