Author/Authors :
Imelda, F. Bogor Agricultural University, Kampus IPB Darmaga - Faculty of Agricultural Engineering and Technology - Department of Food Science and Technology, Food Science Study Program, Indonesia , Imelda, F. Pontianak State Polytechnic - Department of Agricultural Technology, Indonesia , Faridah, D. N. Bogor Agricultural University, Kampus IPB Darmaga - Faculty of Agricultural Engineering and Technology, Southeast Asia Food and Agricultural Science and Technology Center - Department of Food Science and Technology, Food Science Study Program, Indonesia , Kusumaningrum, H. D. Bogor Agricultural University, Kampus IPB Darmaga - Faculty of Agricultural Engineering and Technology, Southeast Asia Food and Agricultural Science and Technology Center - Department of Food Science and Technology, Food Science Study Program, Indonesia
Abstract :
Leaves of Polygonum minus Huds. contain some bioactive compounds which are potential as natural antibacterial agent. The performance of two extraction techniques, i.e. conventional maceration and ultrasound assisted extraction (UAE), for extraction of bioactive compounds of P. minus leaves were evaluated. The antibacterial activities of the extracts were also evaluated towards Escherichia coli and Staphylococcus aureus by agar disc diffusion in combination with TLC-bioautography and by observing the cell membrane leakage. Extraction with UAE provided higher extraction yields and reduced the extraction time in comparison to the conventional maceration. Ethanolic extract of P. minus showed the strongest inhibitory effect for both E. coli and S. aureus. The Minimal Inhibitory Concentration (MIC) of the ethanolic extract, determined by macro-dilution method, was 25 mg/mL for E. coli and 30 mg/mL for S. aureus. TLC-bioautography detected that fractions of ethanolic extract of P. minus with Rf4 = 0.30 and Rf5 = 0.37 demonstrated the most noticeable antibacterial activity for both E. coli and S. aureus. Leakage of cytoplasmic membrane was observed, indicated by the release of cells materials, measured at 260 and 280 nm using UV - Vis spectrophotometer.
Keywords :
Antibacterial activity , Polygonum minus Huds. , TLC , bioautographyUltrasound assisted extraction (UAE)
