Title of article :
Applicability of RIDA®QUICK Verotoxin/O157 Combi kit for detection of Shiga toxin producing Escherichia coli O157:H7 in raw milk
Author/Authors :
Kamal, R. M. Zagazig University - Faculty of Veterinary Medicine - Food Control Department, Egypt , Merwad, A. M. Zagazig University - Faculty of Veterinary Medicine - Department of Zoonoses, Egypt , Ali, S. A. Kuwait University - College for Women - Department of Food Science and Nutrition, Kuwait , Saber, T. M. Zagazig University - Faculty of Medicine - Department of Medical Microbiology and Immunology, Egypt , Bayoumi, M. A. Zagazig University - Faculty of Veterinary Medicine - Food Control Department, Egypt
Abstract :
Many methods have been developed for the rapid and sensitive detection of Escherichia coli O157:H7 in milk and other foodstuffs. This study was designed to evaluate the application of RIDA®QUICK Verotoxin/O157 Combi kit, an immuno-chromatographic detection method, for the detection of this pathogen in raw milk. RIDA®QUICK kit showed appreciable results in detecting Escherichia coli O157:H7 in raw milk, as it was able to detect as low as 100 CFU/ml of raw milk, and as low as 10 CFU/ml of sterilized milk. Raw milk’s microflora negatively affected the detection sensitivity, which could be overcome through a prior antibiotics’ enrichment step. Comparable results between this new method and a reference detection method were also obtained. Obtained data suggests the applicability of RIDA®QUICK kit in detection of Escherichia coli O157:H7 in raw milk.
Keywords :
E. coli O157:H7 , Shiga toxin , Raw milk , PCR , Immunochromatography
Journal title :
International Food Research Journal
Journal title :
International Food Research Journal
