Author/Authors :
Yahya Brawijaya University - Fisheries and Marine Science Faculty - Department of Technology Fishery, Indonesia , Happy, N. Brawijaya University - Fisheries and Marine Science Faculty - Department of Technology Fishery, Indonesia , Yenny, R. Brawijaya University - Fisheries and Marine Science Faculty - Department of Technology Fishery, Indonesia , Soemarno Brawijaya University - Agriculture Faculty, Indonesia
Abstract :
This research is aims to characteritisc of coastal ecosystem bacteria with treatment crude metabolic extraction, purification of crude metabolic with precipitation, dialysis and treatment FT-IR. This precipitate is applied on substance L-histidine becomes histamine on In vitro system. Research method is explorative with parameter which is bacteria isolation test, biochemical test with microbact identification system and application bacteria on L-Histidine with aeration time that is utilized, namely 6 hours, 12 hours, 18 hours, and 24 hours to test histamine rates. This research result extracelluler metabolics bacteria Planococcus citreus on aeration 24 hour point out to histamine rate 26.72 mg/kg, Acinetobacter baumanii 22.75 mg/kg, Enterobacter gergoviae 7.31 mg/kg, Bacillus megaterium 6.20 mg/kg and Nitrococcus sp 2.33 mg/kg respectively. The amount of protein was estimated from metabolic, precipitate and dialisat of bacteria for Planococcus citreus, Enterobacter gergoviae, Acinetobacter baumanii and Bacillus megaterium, showed that is Planococcus citreus beginning 3.27 mg/ml (metabolic crude), 6.65 mg/ml (precipitate 30%), 7.64 mg/ml (precipitate 40%), 8.11 mg/ml (precipitate 50%), 9.25 mg/ml (precipitate 60%), 12.17 mg/ml (precipitate 70%) and 15.57 mg/ml (dialysis purification). The FT-IR spectrum of the protein of Planococcus citreus revealed the presence of 5 peaks at the wave numbers of 345.34, 2075.26, 1639.38, 1400.22 and 1105.14 cm^-1 that indicated the presence of their bending mode amide (N-H), alkena (C=C), imine (C=N), alkana secundere (-CH2-) and carboxyl.
