Author/Authors :
Wihastuti, Titin A. Departement of Biomedicine - Faculty of Medicine - Brawijaya University, Malang, Indonesia , Aini, Fitria N. Departement of Biomedicine - Faculty of Medicine - Brawijaya University, Malang, Indonesia , Lutfiana, Nurul C. Departement of Biomedicine - Faculty of Medicine - Brawijaya University, Malang, Indonesia , Heriansyah, Teuku Departement of Cardiology - Faculty of Medicine - Syiah Kuala University, Aceh, Indonesia , Zamrudah, Nafisatuz Departement of Biomedicine - Faculty of Medicine - Brawijaya University, Malang, Indonesia
Abstract :
Objective:
This study is aimed to examine the expression of ICAM-1 and VCAM-1 in cardiac tissue of dyslipidemic Sprague Dawley rats.
Methods:
Eight Sprague Dawley strain rats, with 150-200 gram body weight, were divided into two groups. The control group was fed a standard diet, the positive control group was fed a high-fat diet as our previous study for 8 weeks. The pattern of distribution of ICAM-1 and VCAM-1 in cardiac muscle cell was examined by immunofluorescence and observed with a confocal laser scanning microscope. Lipid profile was also examined at the end of the study.
Result:
Independent t-test showed no differences in ICAM-1 and VCAM-1 expression in cardiac muscle of hypercholesterol-diet-fed Sprague Dawley rat compared to control.
Conclusion:
The expression of ICAM-1 and VCAM-1 in cardiac muscle did not change after the onset of atherosclerosis.
Keywords :
ICAM-1 , VCAM-1 , Dyslipidemia , Molecule expression , Adhesion , Atherosclerosis , Immunofluorescence
