Title of article :
Detection of Porcine Circovirus Type 2 (PCV2) in Mosquitoes from Pig Farms by PCR
Author/Authors :
Yang, Xiaohong Huazhong Agricultural University - College of Veterinary Medicine - State Key Laboratory of Agricultural Microbiology and Laboratory of Animal Virology, China , Hou, Lidan Huazhong Agricultural University - College of Veterinary Medicine - State Key Laboratory of Agricultural Microbiology and Laboratory of Animal Virology, China , Ye, Jing Huazhong Agricultural University - College of Veterinary Medicine - State Key Laboratory of Agricultural Microbiology and Laboratory of Animal Virology, China , He, Qigai Huazhong Agricultural University - College of Veterinary Medicine - State Key Laboratory of Agricultural Microbiology and Laboratory of Animal Virology, China , Cao, Shengbo Huazhong Agricultural University - College of Veterinary Medicine - State Key Laboratory of Agricultural Microbiology and Laboratory of Animal Virology, China
Abstract :
To investigate, whether mosquitoes could be potential vectors that maintain or transmit PCV2, 59 mosquito samples were collected from suspicious PCV2-infected pig farms in Hubei and Anhui province, China. Total DNA from these mosquitoes was extracted and then tested for presence of PCV2 nucleic acid by PCR. Four (6.78%) samples showed the positive result. Subsequently, the positive PCR product was cloned into pEASY-T1 vector and sequenced. Sequence analysis displayed that homology between the PCR products and PCV2 strains were more than 94%. These results demonstrate that PCV2 nucleic acid exists in these mosquitoes, which suggests that mosquitoes could serve as mechanical transmission vectors of PCV2.
Keywords :
Mosquitoes , PCV2 , Polymerase chain reaction , (PCR) , Vector
Journal title :
Pakistan Veterinary Journal
Journal title :
Pakistan Veterinary Journal
