Title of article :
Comparison of Streptokinase Activity from Streptococcus mutans using Different Substrates
Author/Authors :
Zia, Muhammad Anjum University of Agriculture - Department of Chemistry and Biochemistry, Pakistan , Faisal, Rana University of Agriculture - Department of Chemistry and Biochemistry, Pakistan , Abbas, Rao Zahid University of Agriculture - Department of Parasitology, Pakistan , Gull-e-Faran University of Agriculture - Department of Chemistry and Biochemistry, Pakistan , Saleemi, Muhammad Kashif University of Agriculture - Department of Pathology, Pakistan , Khan, Junaid Ali University of Agriculture - Department of Physiology and Pharmacology, Pakistan
Abstract :
Streptokinase is a novel bacterial fibrinolytic enzyme that binds and activates plasminogen and is produced by several species of Streptococci. Streptococcus mutans was selected for optimum production of streptokinase using corn steep liquor, molasses, rice polishing and sugarcane bagass in liquid state fermentation. Substrates were applied in different concentrations ranging from 0.1-0.8%. Maximum fibrinolytic activity was observed by 0.3% corn steep liquor, 0.5% molasses and rice polishing and 0.4% by sugarcane bagass. The fibrinolytic activity achieved by fibrin clot lysis method was 5.5, 5.08, 5.16 and 4.75 units using corn steep liquor, molasses, rice polishing and sugarcane bagass, respectively.
Keywords :
Corn steep liquor , Streptococcus mutans , Streptokinase , Substrates
Journal title :
Pakistan Veterinary Journal
Journal title :
Pakistan Veterinary Journal
