DEVELOPMENT OF MICROPROPAGATION PROTOCOL FOR MEDICINALLY IMPORTANT PERIPLOCA HYDASPIDIS

The present study represents protocol optimization for in vitro micropropagation of Periploca hydaspidis a weed which is medicinally important. The plant is locally used as purgative. The induction of the shoots was carried out from nodal shoot explants using Murashige and Skoog (MS) media added with different concentrations and combiantions of benzyalminopurine (BAP) and Nephthalene acetic acid (NAA). The effect of different concentrations of BAP and NAA on shoot number, shoot length and per cent shoot induction of explant was noted. The sprouting of shoots started within 7-8 days of inoculation. The combination of BAP 4 mg/L and NAA 1 mg/L induced maximum shoot length (6.25 cm) while minimum induction was noted in combination of BAP 2 mg/L and NAA 0.1 mg/L (1.75 cm). The maximum rooting (4.25 cm) was shown by the combination of IBA 3mg/L and NAA 1.5 mg/L while minimum (1.90 cm) was exhibited by the combination of IBA 2mg/L and NAA 1 mg/L. In vitro micropropagated small plants having healthy roots were transferred to soil through successive acclimatization, which yielded 80 per cent survival rate.