Title of article
Molecular identification of nontuberculous mycobacteria isolated from pyogenic bovine tissues in South Darfur State and Alsabalouga slaughterhouse at Omdurman area, Sudan
Author/Authors
El Tigani-Asil, A.E. Qassim University - Faculty of Agriculture and Veterinary Medicine - Departments of Veterinary Medicine, Saudi Arabia , EL Sanousi, S.M. University of Khartoum - Faculty of Veterinary Medicine - Departments of Microbiology, Sudan , Aljameel, M.A. National Veterinary Research Corporation - Department of Pathology, Veterinary Research laboratory - Nyala, Sudan , El Beir, H. Ministry of Science and Technology - Institute of Tropical Diseases - Department of Tuberculosis, Sudan , Adam, A. University of Nyala - Faculty of Veterinary Science - Department of Pathology, Sudan , Abdallatif, M.M. North Border University - Faculty of Science - Department of Microbiology, Saudi Arabia , Hamid, M.E. King Khalid University - College of Medicine - Department of Clinical Microbiology and Parasitology, Saudi Arabia
From page
16
To page
19
Abstract
This study identified nontuberculous mycobacteria (NTM) recovered from bovine pyogenic affections obtained at necropsy using the molecular target 16S-23S rDNA internal transcribed spacer region. Postmortem inspection of cattle was conducted at South Darfur State abattoirs and Alsabalouga Slaughterhouse at Omdurman area during 2007-2009. Specimens were examined for the presence of acid fast bacteria (AFB) using microscopic and standard culturing techniques. AFB were identified phenotypically and confirmed by 16S-23S rDNA ITS. Fifty nine NTM were recovered and confirmed as acid fast filaments out of 165 positive AFB specimens, of which 52 isolates were identified as bovine farcy causative agents, while 7 cultures were excluded due to drying. 16S-23S rDNA ITS of NTM revealed three different amplicons 500 bp. (32) isolates, 550 bp. (2) isolates and 600 bp. (14) isolates. Four isolates were contaminated.
Keywords
Acid fast bacteria , Bovine farcy , Nontuberculous mycobacteria , Pyogenic infection
Journal title
Open Veterinary Journal
Journal title
Open Veterinary Journal
Record number
2564302
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