Author/Authors :
Saltanatpouri, Zohreh mazandaran university of medical sciences - Sari Medical School - Department of Mycology and Parasitology, ايران , Saltanatpouri, Zohreh tehran university of medical sciences tums - Hematology-Oncology and Stem Cell Transplantation Research Center, تهران, ايران , Shokohi, Tahereh mazandaran university of medical sciences - Sari Medical School - Department of Mycology and Parasitology, ايران , Hashemi Soteh, Mohammad Bagher mazandaran university of medical sciences - Sari Medical School - Department of Biochemistry and Genetics, ايران , Hedayati, Mohammad Taghi mazandaran university of medical sciences - Sari Medical School - Department of Mycology and Parasitology, ايران
Abstract :
Introduction: Candida dubliniensis and C. albicans are very similar in morphology and phenotypic characteristics. Approximation of this yeast species has caused major problems in identifying these two correctly.Materials and Methods: To distinguish among sixty yeast clinical isolates from patients with cancer, polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP) was done.Results: PCR-RFLP of the ITS region showed different patterns between Candida dubliniensis and C. albicans after digestion with enzymes BlnI. All of the Clinical isolates were diagnosed as C .albicans. The results were confirmed by sequencing and RAPD-PCR.Conclusion: PCR-RFLP would be a useful and applicable technique in clinical laboratories for discrimination of C. albicans and C. dubliniensis.
Keywords :
PCR- RFLP , C .dubliniensis , C. albicans , BlnI
