Title of article :
Isolation of a putative invertase gene from the xerophilic Aspergillus niger GH1 strain
Author/Authors :
Veana, F. Autonomous University of Coahuila - School of Chemistry - Department of Food Science and Technology, México , Rodríguez-Reyna, D. G. Autonomous University of Nuevo Leon - School of Biology - Microbiology and Immunology Department, México , Aréchiga-Carvajal, E. T. Autonomous University of Nuevo Leon - School of Biology - Microbiology and Immunology Department, México , Aguilar, C. N. Autonomous University of Coahuila - School of Chemistry - Department of Food Science and Technology, México , Rodríguez-Herrera, R. Autonomous University of Coahuila - School of Chemistry - Department of Food Science and Technology, México
Abstract :
Invertase catalyzes the hydrolysis of sucrose into glucose and fructose and represents a biocatalyst of great importance in food industry. Some xerophilic fungal strains have a great potential to increase enzymatic production. In the present study, five xerophilic fungal strains (Penicillium pinophilum EH2, P. purpurogenum GH2, P. citrinum ESS, Aspergillus niger GH1 and A. fumigatus GS) previously reported as invertase producers were used for isolation and cloning of an invertase gene. A fragment of 1770 bp was isolated and cloned, which corresponding to invertase gene from A. niger GH1. On the other hand, multi-banding was observed from invertase gene from other strains and invertase gene could not be isolated. Results suggested that invertase gene and amino acid sequence exhibited 97% and 99% similarity to invertase from A. niger B60 and A. kawachii IFO 4308, respectively. Therefore, A. niger GH1 could be a good alternative for invertase production.
Keywords :
β , D , fructofuranosidase , cloning , fructooligosacharides , Aspergillus niger , Penicillium.
