Title of article :
In vitro antagonism of Bacillus strains against Fusarium species
Author/Authors :
francisco, nazario francisco universidad autónoma agraria antonio narro - department of parasitology, Mexico , morales, gabriel gallegos universidad autónoma agraria antonio narro - department of parasitology, México , fuentes, yisa maria ochoa universidad autónoma agraria antonio narro - department of parasitology, México , ortiz, juan carlos delgado universidad autónoma agraria antonio narro - department of parasitology, México , castillo, francisco daniel hernandez universidad autónoma agraria antonio narro - department of parasitology, México
Abstract :
Antagonist strains of Bacillus subtilis, B. amyloliquefaciens, B. liquefaciens and B. pumilus were evaluated for their ability to control Fusarium verticilliodes, F. oxysporum, F. acuminatum, F. solani, F. proliferatum and F. oxysporum f. sp. melonis isolated from garlic (Allium sativum L.), melon (Cucumis melo L.), and pepper plants (Capsicum annuum L.). To evaluate the antagonistic effect of different Bacillus species, mycelia plugs from the edges of actively growing fungal cultures were placed in the center of Petri dish containing potato dextrose agar. Bacillus species were inoculated at an equidistant cardinal point. After 168 h, strong growth inhibition was observed with B. pumilus (38% with F. verticilliodes). However F. oxysporum isolated from pepper plant were less inhibited (7-9%) for the four Bacillus species. Fusarium growth inhibition by B. pumilus was evident after 120 h in dual culture.
Keywords :
Bacillus pumilus , Biological control , Root disease
