Author/Authors :
hayat, mian khizar quaid-i-azam university - department of biotechnology, Pakistan , hayat, mian khizar lanzhou university - school of life sciences - moe key laboratory of cell activities and stress adaptations, China , kumar, tanweer lanzhou university - college of pastoral agriculture science and technology - state key laboratory of grassland agro-ecosystems, China , kumar, tanweer national agricultural research centre - parc institute for advanced studies in agriculture (piasa), Pakistan , ahmad, nazir national agricultural research centre - parc institute for advanced studies in agriculture (piasa), Pakistan , jan, sohail ahmad quaid-i-azam university - department of biotechnology, Pakistan , jan, sohail ahmad national agricultural research centre - parc institute for advanced studies in agriculture (piasa), Pakistan , sajjad, wasim quaid-i-azam university - faculty of biological sciences - department of microbiology, Pakistan , faisal, shah lanzhou university - school of life sciences - moe key laboratory of cell activities and stress adaptations, China , faisal, shah chinese academy of sciences - institute of geology and geophysics - key laboratory of petroleum resources, China , gao, bo-xuan lanzhou university - school of life sciences - moe key laboratory of cell activities and stress adaptations, China , khan, khakemin lanzhou university - department of chemistry, state key laboratory of applied organic chemistry, key laboratory of nonferrous metals chemistry and resources utilization of gansu province, China , zia ur rahman, * fujian agriculture and forestry university - center for plant genomics and biotechnology, haixia institute of science and technology, China , abbasi, bilal haidar quaid-i-azam university - department of biotechnology, Pakistan
Abstract :
The current research aimed to evaluate in vitro antimicrobial activity of crude extract prepared from leaves of Aloe vera L. The extract was prepared in different solvents including methanol and ethanol. The inhibitory potential of extract was determined via agar well diffusion method against diverse range of microbes including Erwinia carotovora, Escherichia coli, Klebsiella pneumoniae, Salmonella typhi, Bacillus subtilis, Bacillus cereus, Staphylococcus aureus and Candida albicans. The extracts were applied at 30 μL and 60 μL concentrations. The zone of inhibition was measured and compared with three standards i.e. Clotrimazole (50 μg mL^-1) for Candida albicans; Ciprofloxacin (30 μg mL^-1) for Gram negative bacteria and Azithromycin (50 μg mL^-1) for Gram positive bacteria. The antimicrobial susceptibility test showed that leaf and gel extracts inhibited the growth of all the tested microorganisms while the gel has significantly retarded the growth of E. carotovora, E. coli, B. subtilis and S. aureus. Furthermore, the gram positive strains were inhibited up-to larger extent as compared to gram negative one due to absence of lipopolysaccheride layer in cell wall. The higher inhibitory effect was observed for rotary dried gel methanol extract dissolved in water against C. albiacans at both 30 μL and 60 μL (78%, 104%), respectively. Our results suggest that these herbal extracts may be used against bacterial and fungal pathogens which possesses resistant to conventional antibiotics.
