Author/Authors :
Azarpira, N. shiraz university of medical sciences - Shiraz Transplant Research Center, ايران , Geramizadeh, B. shiraz university of medical sciences - Shiraz Transplant Research Center, ايران , Nikeghbalian, S. shiraz university of medical sciences - Shiraz Transplant Research Center, Organ Transplant Center, ايران , Bahador, A. shiraz university of medical sciences - Shiraz Transplant Research Center, ايران , Yaghobi, R. shiraz university of medical sciences - Shiraz Transplant Research Center, ايران , Karimi, H. shiraz university of medical sciences - Shiraz Transplant Research Center, ايران , Ayatolahi, M. shiraz university of medical sciences - Shiraz Transplant Research Center, ايران , Aghdai, M. H. shiraz university of medical sciences - Shiraz Transplant Research Center, ايران , Salahi, H. shiraz university of medical sciences - Organ Transplant Center, ايران , Malek- Hosseini, S. A. shiraz university of medical sciences - Shiraz Transplant Research Center, Organ Transplant Center, ايران , Roozbeh, J. shiraz university of medical sciences - Organ Transplant Center, ايران , Sagheb, M. shiraz university of medical sciences - Organ Transplant Center, ايران , Raisjalali, G. H. shiraz university of medical sciences - Organ Transplant Center, ايران , Behzadi, A. shiraz university of medical sciences - Organ Transplant Center, ايران
Abstract :
Background: Nitric oxide (NO) is a major mediator in vascular biology, regulating regional blood flow. No and the enzymes required for its production contribute to ischemia-reperfusion injury. The T-786C functional polymorphism in the promoter region substantially reduces promoter activity of the endothelial nitric oxide synthase (eNOS) gene and compromises endothelial NO synthesis. Objective: To examine the association between T-786C (rs 2070744) single nucleotide polymorphism (SNP) in eNOS gene and the development of acute rejection in renal transplant patients. Methods: 60 renal transplant recipients (30 with episodes of acute rejection (ARs) and 30 without ejection (non-ARs)), between June 2008 and March 2010, were included in this study. The polymorphism as determined by PCR-restriction fragment-length polymorphism analysis. Results: The distribution of the genotypes were TT/TC/CC 60%, 33.4%, 6.6%, and 43%, 46.7%, 3.3% in ARs and non-ARs, respectively (p=0.28). The frequency of T-allele was 76.7% and 66.3%; and for C-allele was 66.6% and 33.3% in ARs and non-ARs, respectively (p=0.09). There were no significant associations between these polymorphisms and acute and chronic kidney allograft rejection. Conclusion: We could not detect any significant association between polymorphism in T-786C of NOS gene and the development of acute rejection
Keywords :
Nitric oxide , Endothelial nitric oxide synthase , Polymorphism , Transplant , Acute rejection
