Human Leukocyte Antigen-G Expression on Dendritic Cells Induced by Transforming Growth Factor-βl and CD4+ T Cells Proliferation

Background: During antigen capture and processing, mature dendritic cells (DC) express large amounts of peptide-MHC complexes and accessory molecules on their surface. DC are antigen-presenting cells that have an important role in tolerance and autoimmunity. The transforming growth factor-beta 1 (TGF-pi) cytokine has a regulatory role on the immune and non-immune cells. The aim of this study is to evaluate the effect of TGF-pl on the induction of human leukocyte antigen-G (HLA-G) expression on the DC which is derived from monocyte. Methods: In this study, we evaluated the effect of TGF-pi in induction HLA-G expression on the monocyte-derived DC by flowcytometry and then CD4+ T cell proliferative responses in the presence of DC-treated TGF-pi was studied. Results: The results of this study showed that DC bearing HLA-G down- regulated activation of CD4+ T cells and production of IL-6 and IL-17 in comparison with control (P0.05). Conclusion: It is concluded that TGF-pi has an important regulatory role in CD4+ T cell proliferation by increasing HLA-G on DC and these cells can probably prevent unexpected immune responses in vivo. Iran. Biomed. J. 15 (1 2): 1-5, 2011