Author/Authors :
Aligholi, Marzieh tehran university of medical sciences tums - School of Medicine - Department of Microbiology, تهران, ايران , Emaneini, Mohammad tehran university of medical sciences tums - School of Medicine - Department of Microbiology, تهران, ايران , Jabalameli, Fereshteh tehran university of medical sciences tums - School of Medicine - Department of Microbiology, تهران, ايران , Shahsavan, Shadi tehran university of medical sciences tums - School of Medicine - Department of Microbiology, تهران, ايران , Dabiri, Hosein tehran university of medical sciences tums - School of Medicine - Department of Microbiology, تهران, ايران , Sedaght, Hossein tehran university of medical sciences tums - School of Medicine - Department of Microbiology, تهران, ايران
Abstract :
Objective: The objective of the study was to investigate the prevalence of vancomycin-resistant Staphylococcus aureus (VRSA). Materials and Methods: Three hundred and fifty-six S. aureus isolates from the Imam Khomeini hospital in Tehran, Iran, were evaluated for methicillin and decreased vancomycin susceptibility by the microbroth dilution method. The mecA, vanAand vanB genes were targeted by polymerase chain reaction. Results: Of the 356 isolates, 149 (41.85%) S. aureus strains were resistant to methicillin. Two strains of methicillin-resistant S. aureus were VRSA strains. One isolate, Teaching Hospital-1 (TEH-1), had a vancomycin minimum inhibitory concentration (MIC) of 64 μg/ml and was susceptible to teicoplanin while the other isolate (TEH-2) had a vancomycin and teicoplanin MIC of 512 and 256 μg/ml, respectively, and was positive for the vanA gene. Conclusion: This report shows that the emergence of VRSA in Iran warrants active microbiological surveillance and careful monitoring of vancomycin therapy.
Keywords :
Methicillin , resistant Staphylococcus aureus , Vancomycin , Vancomycin , resistant Staphylococcus aureus , vanA gene
