Title of article
Cytotoxic Effect of Capparis spinosa L. on PLC/PRF/5 Human Hepatocellular Carcinoma Cell Line
Author/Authors
sheikh, masoud islamic azad university, science and research branch - college of veterinary science - department of veterinary basic science, ايران , eshraghi, hamid reza islamic azad university, science and research branch - college of veterinary science - department of veterinary basic science, ايران , khoshnia, masoud golestan university of medical sciences - golestan research center of gastroenterology and hepatology-grcgh, ايران , mazandarani, masoumeh islamic azad university, gorgan branch - department of botany, ايران , moradi, abdolvahab golestan university of medical sciences - golestan research center of gastroenterology and hepatology-grcgh, ايران
From page
9
To page
12
Abstract
Background and Objectives: Capparis spinosa has been used in traditional medicine for various applications including treatment of liver disorders and cancer. We studied the effects of this plant on cell proliferation and morphological characteristics of PLC/PRF/5 liver cancer cell line. Methods: After preparing ethanolic extract of the plant, the inhibitory effect of the extract was assessed using MTT assay, and morphological changes were assessed by an inverted microscope. Results: C. spinosa ethanolic extract exhibited anti-cancer effects in a concentrationdependent manner. Half-maximal inhibitory concentration of the extract was 1051±4.21 mg/mL. Morphological changes including cell shrinkage, reduction of cell volume and nuclear condensation confirmed the inhibitory effect of C. spinosa on PLC/PRF/5 cells. Conclusion: According to the results of this study, extract of C. spinosa seems to be suitable for prevention and treatment of liver cancer. Further studies on animal models could verify the efficiency of the extract against cancer cells.
Keywords
Plants , Medicinal , Capparis spinosa , Liver Neoplasms
Journal title
Medical Laboratory Journal
Journal title
Medical Laboratory Journal
Record number
2567962
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