Author/Authors :
dashti, mohsen mashhad university of medical sciences (mums) - ghaem hospital - blood banking laboratory, ايران , bahrami, afsane mashhad university of medical sciences - school of medicine - department of modern sciences and technologies, ايران , sadeghian, mohammad hadi mashhad university of medical sciences - cancer molecular pathology research center, ايران , shams, fatemeh mashhad university of medical sciences - cancer molecular pathology research center, ايران , ashjaee, ahmad mashhad university of medical sciences (mums) - ghaem hospital - blood banking laboratory, ايران , arianpour, zahra iranian blood transfusion organization research center, ايران , shakeri, sepideh mashhad university of medical sciences - cancer molecular pathology research center, ايران
Abstract :
Background and Objectives: Blood transfusion may induce some adverse effects on receivers. Some methods such as antibody screening and cross matching have been suggested to reduce the risk of transfusion complications. However, these methods require commercial antibody screening kits that may also need special equipment. The aim of this study was to introduce a new method for antibody screening that does not require a commercial kit, and could be used in any transfusion laboratory. Methods: We examined 350 samples that contained alloantibody and 350 control samples without the antibody. A solution containing two O+ and one O- samples were used instead of screening cells. Results: Sensitivity and specificity of the method were 73.32% and 45.15%, respectively. Positive predictive value and negative predictive value were 58.33% and 63.88%, respectively. Conclusion: Our new method can be used in basic hematology laboratories with some modifications.
