Author/Authors :
Shah, Shaukat A Postgraduate Medical Institute (PGMI) - Department of Pathology, Pakistan , Shah, Shaukat A Sir Ganga Ram Hospital, Pakistan , Shariff, Mian M. Fatima Jinnah Medical College, Pakistan , Shariff, Mian M. Zeenat Medical Laboratories, Pakistan , Shariff, Mian M. National Hospital, Pakistan , Shariff, Mian M. Sir Ganga Ram Hospital, Pakistan , Khan, Saeed A Postgraduate Medical Institute (PGMI), Pakistan , Tayyab, Muhammad Postgraduate Medical Institute (PGMI), Pakistan , Chaudary, Naseer A Postgraduate Medical Institute (PGMI), Pakistan , Ahmed, Nazifa Fatima Jinnah Medical College - Pathology department, Pakistan , Ahmed, Nazifa Sir Ganga Ram Hospital, Pakistan
Abstract :
Objective: To correlate blood lead level with the atresia of ovarian follicles of albino mice following exposure to different doses of lead. Material and Methods: This experimental Study was carried out at Postgraduate Medical Institute, Lahore from 1st February to 31st March, 2004. A total of 40 adult virgin female albino mice of 6–8 weeks age weighing between 30 ± 2 grams were divided randomly into four groups of 10 each. Group I was not given lead acetate, whereas group II, group III and group IV were given lead acetate in doses 2, 4, 8 mg mg /kg/ day, respectively for 60 days by oral lavage. Blood samples were collected in EDTA tubes for the estimation of lead level. Both ovaries were removed by dissection and processed with the standard histopathological technique for the quantitative assessment of follicle numbers in response to toxic effects of lead. Results: Blood lead levels were the lowest in group Id (0.16μg/ml mean SD 0.18 ± 2) and highest in group IV (0.62μg/ml mean ± SD 0.60 ± 0.015) and revealed significant statistical difference (P 0.001) between groups I vs II , I vs III and I vs IV. Lead levels were found to be elevated with the increment in the dose of lead acetate. The counting of various stages of atretic follicle in the control group I and treated group II, group III and group IV of albino mice showed that as the blood lead levels increased, the percentage number of atretic follicles also increased as shown in the medium follicles of the control group I: 19.4%, group II: 29.7%, group III: 44.4% and group IV: 69.6% , whereas, the large follicles control group I revealed 4.8%, group II 9.4%, group III 17.4% and group IV 29.1%. Our results indicated that artesia of medium sized follicles reflected the extent of damage caused by the lead. Both large and medium sized follicles demonstrated a highly significant correlation (r = 0.91 and 0.97, p 0.001) with the blood lead level respectively. Conclusion: Oral administration of lead in high doses leads to reduction in the number of ovarian follicles. Our data revealed a strong correlation between blood lead level and atresia of ovarian follicles of albino mice which is consistent with the literature
Keywords :
Blood lead levels , Mice , Correlation , Atresia , Ovarian follicles
