Author/Authors :
romani, s. shahid beheshti university of medical sciences, تهران, ايران , azimzadeh, p. shahid beheshti university of medical sciences, تهران, ايران , mohebbi, s.r. shahid beheshti university of medical sciences, تهران, ايران , bozorgi, s.m. shahid beheshti university of medical sciences, تهران, ايران , zali, n. shahid beheshti university of medical sciences, تهران, ايران , jadali, f. shahid beheshti university of medical sciences, تهران, ايران
Abstract :
Aim: This study investigated the prevalence of sapovirus infections in patient with acute gastroenteritis in Tehran, Iran. Background: Sapovirus, a member of the family Caliciviridae is one of the major causative agents of viral gastroenteritis affecting both children and adult individuals. There isn t enough data about prevalence and genotypes of sapovirus infection in Tehran, the capital city of Iran. Patients and methods: A total of 42 fecal samples were collected from patients with acute gastroenteritis from May to July 2009. RT nested- PCR was performed for screening. To genotype the sapovirus isolates, some positive samples were subjected to phylogenetic analysis by sequencing of fragments of viral capsid gene region. Results: Sapovirus was detected in 5 of 42 stool specimens from patients with acute gastroenteritis. Sapovirus detected in this study was clustered into only one distinct genogroup I/2. Sapovirus GI/2 was predominant. Conclusion: Our results show that among the studied viruses responsible for this disease, sapovirus was a major viral isolate virus. © 2012 RIGLD.
