Author/Authors :
Hashmat Ullah Gomal Univeristy - Faculty of Pharmacy, Pakistan , Khan, Muhammad Farid Gomal University - Faculty of Pharmacy, Pakistan , Jan, Syed Umer University of Balochistan - Department of Pharmacy, Pakistan , Shaikh, Ubedullah Dow University Hospital - Department of Surgery, Pakistan , Hashmat, Farwa Gomal University - Department of Chemistry, Pakistan , Ali, Muhammad Zeeshan Gomal University - Gomal Center of Biochemistry and Biotechnology, Pakistan , Khan, Habibullah Gomal Medical College - Department of Medicine, Pakistan
Abstract :
Background: Environmental pollution of transition metals especially of mercury is of great concern to the researchers in medical field since long. Mercury causes oxidative stress in all the cells including lymphocytes of the human immune system. Effect of inorganic compound of mercury was compare with our own report of effect of organic compound of mercury on T and B lymphocytes in human blood. Material Methods: The present experimental work was design to analyze the concentration and time dependent effect of mercuric chloride on the chemical modulation of T and B-lymphocytes GSH of human blood. Terasaki technique for lymphocytes isolation and then subsequently separation of T and B-cells was used. Quantification of thiol in T and B-cells was done by Ellman’s method. Results: The lowest used concentration of mercuric chloride depleted T and B-cells GSH level 2.115 μM (27.34%) and 2.019 μM (32.41%) respectively which was significant (p 0.001) decrease in both T and B-cells GSH level. This effect of mercury was dose and time dependent. Conclusion: This study suggests that depletion of GSH due to inorganic mercury in T and B-lymphocytes is more severe as compared to its organic form
Keywords :
Antioxidant , Mercuric chloride , T , lymphocytes , B , lymphocytes , Pollutants , Lipid peroxidation
