Author/Authors :
VAEZJALALI, MARYAM tehran university of medical sciences tums - Faculty of Public Health - Department of Virology, تهران, ايران , ALAVIAN, SEYED MOAYED baqiyatallah university of medical sciences - Baqiyatallah Research Center for Gastroenterology and Liver Disease, تهران, ايران , JAZAYERI, SEYED MOHAMMAD tehran university of medical sciences tums - Faculty of Public Health - Department of Virology, تهران, ايران , NATEGH, RAKHSHANDEH tehran university of medical sciences tums - Faculty of Public Health - Department of Virology, تهران, ايران , MAHMOODI, MAHMOOD tehran university of medical sciences tums - Faculty of Public Health - Department of Virology, تهران, ايران , HAJIBEIGI, BASHIR Tehran Hepatitis CentER, ايران , MOKHTARI, TALAT tehran university of medical sciences tums - Faculty of Public Health - Department of Virology, تهران, ايران
Abstract :
Background and Aims: Determination of the genotypes and subtypes of hepatitis B virus (HBV) provides epidemiological data, which can contribute further to vaccination and antiviral treatment strategies, diagnostic development, and prediction of the disease course. The aim of this study was to describe the molecular characterization and phylogenetic analysis of 60 HBV S-region isolated from native Iranian patients with chronic HBV infection. Methods: HBV-positive sera were collected from Iranian patients with hepatitis B infection in Tehran Hepatitis Center. HBV-DNA was extracted and the partial HBV S ORF (677bp) were chosen for amplification and sequencing. Results: By comparing the sequences of HBV isolated from Iranian patients with SO complete sequences of HBV retrieved from the GenBank database, representing all other existing genotypes, the sequences of all 60 patients were consistent with that of genotype D. All HBV isolates from Iranian patients were clustered in genotype D branch with high bootstrap values. Conclusions: Sixty HBV isolates from Iranian patients with chronic hepatitis B represent homogenous genotypic diversity. These sequences of Iranian HBV genomes may contribute to the information on the genetic diversity of HBV worldwide.
Keywords :
Hepatitis B Virus , Genotype , Chronic Carrier , Iran
