Author/Authors :
Soufizomorrod, Mina tarbiat modares university - Department of Hematology and Blood banking, تهران, ايران , Soleimani, Masoud tarbiat modares university - School of Medical Sciences - Department of Hematology and Blood banking, تهران, ايران , Hajifathali, Abbas shahid beheshti university - Taleghani Hospital - Department of Medical Oncology and Hematology, تهران, ايران , Mossahebi Mohammadi, Majid tarbiat modares university - Department of Hematology and Blood banking, تهران, ايران , Abroun, Saeed tarbiat modares university - Department of Hematology and Blood banking, تهران, ايران
Abstract :
Umbilical cord Blood (UCB) as a source of Hematopoietic Stem/Progenitor cells (HSPCs) used for Umbilical cord blood transplantation (UCBT). The main obstacle in application of this source as an appropriate source of HSPCs is low volume of this product. So ex vivo expansion of these cells in a microenvironment which mimic body condition is important. In current study we designed biocompatible microwells in which collagene type I is coated by softlitography method. Our findings designated that in 3-Dimensional (3D) microenvironment CD133+ UCB derived HSC expanded significantly compared to 2-Dimensional (2D) microenvironment.
